Effect of Topical Gel Administration of Secretome Hypoxia Mesenchymal Stem Cells on IL-10 and TNF-α Gene Expression: An In Vivo Experimental Study in Male Wistar Rats with Fluconazole-Induced Alopecia

The study investigated the effect of topical SH-MSCs gel on IL-10 and TNF-α gene expression in Wistar rats with fluconazole-induced alopecia. Four groups were used: two treatment groups with SH-MSCs gel doses of 20 μL and 40 μL, a base gel control group, and a healthy control group. Analysis on day 22 showed that SH-MSCs gel significantly increased IL-10 gene expression and decreased TNF-α gene expression, with the 40 μL dose having the most significant effect. This suggests that SH-MSCs gel could be a safe and effective alternative therapy for alopecia.