The Effect of Topical Gel Secretome Hypoxic Mesenchymal Stem Cells on IL-6 and TGF-β Gene Expression in Male Wistar Alopecia-Like Rat Models Induced by Fluconazole

This study investigated the effects of Secretome Hypoxic Mesenchymal Stem Cells (SH-MSCs) on IL-6 and TGF-β gene expression in male Wistar rats with alopecia induced by fluconazole. The rats were divided into four groups: a healthy group, a negative control group, and two treatment groups receiving 10% and 20% SH-MSCs doses in 200 mg/rat/day gel. After 21 days, IL-6 and TGF-β expressions were measured. Results showed that SH-MSCs significantly decreased IL-6 expression (p = 0.000) and increased TGF-β expression (p = 0.000) in the 20% dose group. These findings suggest that SH-MSCs could be a promising and safe alternative therapy for alopecia.