Long-Term Expansion and Differentiation of Adult Murine Epidermal Stem Cells in 3D Organoid Cultures

    June 2019 in “ Proceedings of the National Academy of Sciences ”
    Kim E. Boonekamp, Kai Kretzschmar, Dominique J. Wiener … Hans Clevers
    Studysummary This study introduces a method for long-term expansion of adult mouse epidermal stem cells in vitro using an organoid culture system that maintains the basal-apical organization.
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    Research cited in this study 14

    1. Self-Organization Process in Newborn Skin Organoid Formation Inspires Strategy to Restore Hair Regeneration of Adult Cells Proceedings of the National Academy of Sciences of the United States of America · 2017
    2. Hair Follicle Stem Cell Cultures Reveal Self-Organizing Plasticity of Stem Cells and Their Progeny The EMBO Journal · 2016
    3. Compartmentalized Epidermal Activation of β-Catenin Differentially Affects Lineage Reprogramming and Underlies Tumor Heterogeneity Cell reports · 2016
    4. Markers of Epidermal Stem Cell Subpopulations in Adult Mammalian Skin Cold Spring Harbor Perspectives in Medicine · 2014
    5. Spatial Organization Within a Niche as a Determinant of Stem-Cell Fate Nature · 2013
    6. Regenerating the Skin: A Task for the Heterogeneous Stem Cell Pool and Surrounding Niche Nature Reviews Molecular Cell Biology · 2013
    7. Paracrine TGF-β Signaling Counterbalances BMP-Mediated Repression in Hair Follicle Stem Cell Activation Cell stem cell · 2012
    8. Coordinated Activation of Wnt in Epithelial and Melanocyte Stem Cells Initiates Pigmented Hair Regeneration Cell · 2011
    9. Epidermal Stem Cell Diversity and Quiescence EMBO molecular medicine · 2009
    10. Epidermal Homeostasis: A Balancing Act of Stem Cells in the Skin Nature Reviews Molecular Cell Biology · 2009
    11. Lgr5 Marks Cycling, Yet Long-Lived, Hair Follicle Stem Cells Nature Genetics · 2008
    12. Stem Cells in the Hair Follicle Bulge Contribute to Wound Repair but Not to Homeostasis of the Epidermis Nature Medicine · 2005
    13. Noggin Is Required for Induction of the Hair Follicle Growth Phase in Postnatal Skin The FASEB Journal · 2001
    14. Inhibition of BMP Signaling Affects Growth and Differentiation in the Anagen Hair Follicle The EMBO Journal · 2000