Inhibitory Effect of 7DHC and BM15766 in Hair Follicle Organoid Culture

September 2025 in “ OPAL (Open@LaTrobe) (La Trobe University) ”

Leemon Nikhila (22250736), Suresh Surya (22250739), Shahul Hameed Najeeb (22250742), Thankachan Mangalathettu Binumon (22250745), Aayush Gupta (9580239), Sandeep Gopalakrishnan (334402), Mahsa Dabagh (3972137), Parameswara Panicker Sreejith (22250748)

7DHC BM15766 hair follicle organoid hair follicle bulge SOX9 LGR5 K15 apoptosis keratin filaments connective tissue sheaths

TLDR 7DHC and BM15766 damage hair follicle structure and reduce key gene expression.

The study investigates the effects of BM15766 and 7DHC on hair follicle organoid cultures, focusing on the hair follicle bulge, a key stem cell region. Both compounds caused structural damage, disrupted cellular organization, and led to the deterioration of the hair matrix, pushing the hair fiber upwards. Real-time PCR showed significant down-regulation of SOX9 and LGR5 gene expression in treated samples compared to controls. Immunofluorescence imaging revealed reduced LGR5 and K15 expression and increased apoptosis in treated hair follicles. TEM analysis showed that treated samples had ruptured matrix cells, fragmented keratin filaments, and degraded connective tissue sheaths, indicating significant structural damage compared to well-organized control samples.

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