Development of a Liquid Chromatography/Mass Spectrometry-Based Inhibition Assay for Screening Steroid 5-Alpha Reductase in Human and Fish Cell Lines

February 2021 in “ Molecules ”

Dahye Kim, Hyun-Ki Cho, Ruth Eggers, Sang Hoon Kim, Chang Y. Ryu, Young Ho Kim

Dihydrotestosterone DHT 5-alpha reductase 5AR finasteride dutasteride testosterone steroid metabolism endocrine disruption Propecia Avodart

From the encyclopedia

Finasteride →

Frontline, gold standard treatment for combatting androgenic alopecia

Dutasteride →

Heavy duty finasteride that comes with higher risks

Studysummary In this study, researchers developed a sensitive assay to measure the inhibition of steroid 5-α reductase (5AR) by chemicals like finasteride and dutasteride, revealing species-specific differences in inhibitor efficacy between human and fish cell lines.

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research Identification of a New Plant Extract for Androgenic Alopecia Treatment Using a Non-Radioactive Human Hair Dermal Papilla Cell-Based Assay

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research Determination Method for Steroid 5α-Reductase Activity Using Liquid Chromatography/Atmospheric Pressure Chemical Ionization–Mass Spectrometry

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This study developed a method using LC/APCI-MS to measure the activity of steroid 5α-reductase, applied to kinetic studies and inhibitory tests with Finasteride in a rat prostatic enzyme model.

research The Long-Term Effect of Doxazosin, Finasteride, and Combination Therapy on the Clinical Progression of Benign Prostatic Hyperplasia

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research PNU 157706, A Novel Dual Type I and II 5α-Reductase Inhibitor

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This study found that the pH dependency of rat steroid 5α-reductase type II isozyme significantly affects its kinetic properties, including Vmax and Km, suggesting past discrepancies in literature may arise from these pH variances during assays.

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In this study, the combination of finasteride and minoxidil significantly increased hair growth in balding male stumptail macaques compared to each drug alone.