4-O-Methylhonokiol Protects HaCaT Cells from TGF-β1-Induced Cell Cycle Arrest by Regulating Canonical and Non-Canonical Pathways of TGF-β Signaling
February 2017
in “
Biomolecules & Therapeutics
”
4-O-Methylhonokiol HaCaT cells TGF-β1 cell cycle arrest canonical pathway non-canonical pathway Smad-dependent pathway Smad-independent pathway G1/G0 phase arrest p21 expression nuclear translocation Smad2/3 Smad4 Sp1 reactive oxygen species NADPH oxidase 4 mRNA MH human skin cells TGF-beta1 cell growth inhibition Smad pathway ROS NOX4
TLDR 4-O-Methylhonokiol helps protect skin cells from growth-stopping effects of a protein by regulating growth-related pathways.
The 2017 study found that 4-O-Methylhonokiol (MH), a compound derived from the Magnolia tree, could protect human skin cells (HaCaT cells) from cell cycle arrest induced by TGF-β1, a protein that can inhibit cell growth and potentially lead to conditions like alopecia. MH was found to regulate both the canonical (Smad-dependent) and non-canonical (Smad-independent) pathways of TGF-β signaling, which are involved in cell growth and differentiation. Specifically, MH inhibited TGF-β1-induced cell cycle arrest by decreasing G1/G0 phase arrest and p21 expression, blocking nuclear translocation of Smad2/3, Smad4 and Sp1, and reducing TGF-β1-induced reactive oxygen species production and NADPH oxidase 4 mRNA level. These findings suggest that MH could potentially be used as a therapeutic agent for conditions related to cell cycle arrest, such as hair loss.
