A Quantitative Ultra-Performance Liquid Chromatography High-Resolution Mass Spectrometry Analysis of Steroids from Human Scalp Hair

    June 2022 in “ Journal of pharmaceutical and biomedical analysis ”
    Johanna Koskivuori, Raimo Voutilainen, Lauri Uusitalo, Marko Lehtonen, Timo A. Lakka, Seppo Auriola, Merja R. Häkkinen
    TLDR The method can measure multiple steroids in human hair to study long-term steroid metabolism, especially in newborns and children.
    The document details a study that developed a new method for analyzing multiple steroids from human scalp hair using high-resolution mass spectrometry. This method was tested on 22 steroids and found to be suitable for the quantitative analysis of endogenous glucocorticoids, androgens, progestogens, and estrogens. The method was applied to hair samples from different age groups, including newborns, adolescents, and adults. The results showed that newborn hair contains the widest variety and the highest amounts of steroids compared to other age groups. The method demonstrated good linearity for all analytes in their calibration ranges (r2 ≥ 0.99) and good precision for all the analytes (RSD 1–12%) except for 11KT (RSD 6–16%) and 11KA4 (RSD 11–18%). The method can be further utilized in exploring steroid hormone profiles in different age groups and conditions.
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