A Quantitative Ultra-Performance Liquid Chromatography High-Resolution Mass Spectrometry Analysis of Steroids from Human Scalp Hair

    April 2022 in “ Journal of pharmaceutical and biomedical analysis ”
    Johanna Koskivuori, Raimo Voutilainen, Lauri Uusitalo, Marko Lehtonen, Timo A. Lakka, Seppo Auriola, Merja R. Häkkinen
    TLDR The method can measure multiple steroids in human hair to study long-term steroid metabolism, especially in newborns and children.
    The document details a study that developed a new method for analyzing multiple steroids from human scalp hair using high-resolution mass spectrometry. This method was tested on 22 steroids and found to be suitable for the quantitative analysis of endogenous glucocorticoids, androgens, progestogens, and estrogens. The method was applied to hair samples from different age groups, including newborns, adolescents, and adults. The results showed that newborn hair contains the widest variety and the highest amounts of steroids compared to other age groups. The method demonstrated good linearity for all analytes in their calibration ranges (r2 ≥ 0.99) and good precision for all the analytes (RSD 1–12%) except for 11KT (RSD 6–16%) and 11KA4 (RSD 11–18%). The method can be further utilized in exploring steroid hormone profiles in different age groups and conditions.
    Discuss this study in the Community →

    Research cited in this study

    2 / 2 results

    Related Community Posts Join

    6 / 111 results

      community 2 year fin/min progress (age 27-29)

      in Progress Pictures  302 upvotes 1 year ago
      A user's two-year progress using 1mg of oral finasteride daily and twice daily application of 5% minoxidil solution for hair loss. The user experienced temporary side effects but is pleased with the results, and another user warns about the importance of consistent use for maintaining results.

      community Fin+Colostrum: My journey is a little different.

      in Is this regrowth?  14 upvotes 1 week ago
      The user has been using finasteride for 30 years and recently added colostrum to their routine, noticing darker hair regrowth. They experienced shedding with minoxidil and advise against its use, while highlighting the benefits and side effects of long-term finasteride use.

      community How Dangerous is Oral Min Really?

      in Minoxidil  26 upvotes 1 week ago
      Oral minoxidil is generally effective for hair loss but may cause side effects like heart palpitations and facial puffiness. Users highlight the importance of monitoring blood pressure due to potential cardiovascular risks.

      community Nandrolone Replacement Therapy

      in Treatment  41 upvotes 1 year ago
      A user discusses starting nandrolone replacement therapy to stop hair loss and improve hair health, noting its benefits over finasteride. They report halted hair loss, improved skin, increased muscle, and a calm mood after three weeks.

    Similar Research

    5 / 1000+ results