Study on Dissolution Mechanism of Cortisol and Cortisone from Hair Matrix with Liquid Chromatography–Tandem Mass Spectrometry

March 2013 in “ Clinica Chimica Acta ”

Xing Xue, Chen Zheng, Jifeng Li, Jing Zhang, Huihua Deng, Zuhong Lu

cortisol cortisone hair matrix liquid chromatography–tandem mass spectrometry LC–MS/MS

TLDR Cortisol and cortisone release from hair increases in steps over time during incubation.

The study investigated the dissolution mechanism of cortisol and cortisone from hair matrix using liquid chromatography–tandem mass spectrometry (LC–MS/MS). It focused on the effect of incubation duration in methanol and water on the concentration of these compounds in no-milled and milled hair samples. The results showed that there were 2 or 3 peaks in the concentration of cortisol and cortisone during incubation, indicating a stepwise release from the hair structure. The study highlighted the importance of incubation duration in accurately determining hair cortisol and cortisone levels, with concentrations increasing in a stepwise manner over time.

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