Spatial Transcriptomics of a Giant Pilomatricoma

This study applied 10X Genomics Visium Spatial Gene Expression analysis to a giant pilomatricoma (PM) from a 67-year-old man, revealing five distinct transcriptomic clusters. Four clusters represented the tumor, showing a maturation pattern from basaloid cells to keratinized layers, while one cluster was benign stroma. Unique gene sets were identified in each cluster, with Cluster 1 enriched in DNA replication and cell division processes, Cluster 2 in cell adhesion and skin barrier formation, and Cluster 4 in keratinization and hair cycle processes. The study highlights the potential of spatial transcriptomics in understanding PM pathobiology but notes limitations such as the need for protein-level validation and analysis of additional samples.