Restructuring Skin Equivalents With Human Hair Follicle ORSCs and Fibroblasts

March 2007 in “ Journal of Plastic Reconstructive & Aesthetic Surgery ”

Shanshan Liu, T. Egana, I. Wilcke, Juergen Lohmeyer, Daniel Thomé, Enikő Bodó, Zhongfa Lu, Stefan Krüger, P. Mailänder, Hans-Günther Machens

human dermal fibroblasts collagen-GAG matrix outer root sheath cells hair follicles bulge region multipotent stem cells skin equivalents wound healing skin reconstruction

Studysummary A new method was developed to create better skin models for healing and reconstruction. Our plain-language summary of this paper — not a Tressless recommendation.

The study focused on developing a three-dimensional skin equivalent (SE) model using human dermal fibroblasts, a collagen-GAG matrix, and outer root sheath cells (ORSCs) from hair follicles. The bulge region of the hair follicle, which contains multipotent stem cells, was utilized to improve the quality of the SE. This approach aimed to create a rapid, easy, and effective method for producing skin equivalents, potentially benefiting wound healing and skin reconstruction applications.

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Research cited in this study

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research Characterization and Isolation of Stem Cell-Enriched Human Hair Follicle Bulge Cells

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This study found that bulge cells marked by CD200+ in human hair follicles show high colony-forming efficiency, suggesting successful enrichment of keratinocyte stem cells.

research A Review: The Location, Molecular Characterization and Multipotency of Hair Follicle Epidermal Stem Cells

61 citations , November 2004 in “Annals of the Academy of Medicine Singapore”

This review examines the location and markers of hair follicle stem cells, finding that they are primarily located in the bulge region and can contribute to various skin structures, but challenges remain in distinguishing them from their progeny.

research Human Follicular Stem Cells: Their Presence in Plucked Hair and Follicular Cell Culture

82 citations , May 2004 in “British Journal of Dermatology”

This study found CK19/Bcl-2-positive and Bax-negative cells in hair follicle cultures from plucked hairs, supporting their potential identification as follicular stem cells located in the bulge area.

research An Improved and Rapid Method to Construct Skin Equivalents from Human Hair Follicles and Fibroblasts

48 citations , August 2001 in “Experimental dermatology”

This study developed a rapid, cost-effective three-dimensional skin equivalent model using human dermal fibroblasts, collagen, and hair follicles, which closely resembles natural human skin and can be used for autologous transplantation.

research Involvement of Follicular Stem Cells in Forming Not Only the Follicle but Also the Epidermis

1010 citations , August 2000 in “Cell”

In this study, researchers found that hair follicle stem cells in mice can generate both hair follicle and epidermis cells, highlighting their potential bipotency.

research Characterization of a New Tissue-Engineered Human Skin Equivalent with Hair

221 citations , June 1999 in “In Vitro Cellular & Developmental Biology - Animal”

research Successful Treatment of Chronic Leg Ulcers with Epidermal Equivalents Generated from Cultured Autologous Outer Root Sheath Cells

99 citations , July 1996 in “Journal of Investigative Dermatology”

Using patient's own hair cells can effectively heal chronic leg ulcers.

research Keratin 19 as a Biochemical Marker of Skin Stem Cells In Vivo and In Vitro: Localization and Variation with Age and Culture Stage

441 citations , May 1996 in “Journal of Cell Science”

This study found that keratin 19 may be a marker for skin stem cells, helping to characterize these cells in different conditions and potentially explaining variations in healing rates between children and adults.

research Architecture of Reconstructed Epidermis on Collagen Lattices Varies According to the Method Used: A Comparative Study

9 citations , January 1990 in “Skin Pharmacology and Physiology”

This study observed that 67 kDa keratin was expressed homogeneously only in epidermis derived from hair follicle explants, differing from other culture methods where expression was delayed and patchy.

research Keratin 19: Predicted Amino Acid Sequence and Broad Tissue Distribution Suggest It Evolved from Keratinocyte Keratins

238 citations , May 1989 in “Journal of Investigative Dermatology”