TLDR A new method was developed to create better skin models for healing and reconstruction.
The study focused on developing a three-dimensional skin equivalent (SE) model using human dermal fibroblasts, a collagen-GAG matrix, and outer root sheath cells (ORSCs) from hair follicles. The bulge region of the hair follicle, which contains multipotent stem cells, was utilized to improve the quality of the SE. This approach aimed to create a rapid, easy, and effective method for producing skin equivalents, potentially benefiting wound healing and skin reconstruction applications.
550 citations
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December 2005 in “The Journal of clinical investigation/The journal of clinical investigation” Researchers successfully isolated and identified key markers of stem cell-enriched human hair follicle bulge cells.
61 citations
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November 2004 in “Annals of the Academy of Medicine Singapore” Hair follicle stem cells are mainly in the bulge region and can help repair skin and form hair and glands, but more research is needed to fully understand them.
82 citations
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May 2004 in “British Journal of Dermatology” Stem cells are present in plucked hair follicles and can be maintained in culture.
48 citations
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August 2001 in “Experimental dermatology” Researchers created a quick, cost-effective way to make skin-like tissue from hair follicles and fibroblasts.
1010 citations
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August 2000 in “Cell” Hair follicle stem cells can form both hair follicles and skin.
221 citations
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June 1999 in “In Vitro Cellular & Developmental Biology - Animal” 99 citations
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July 1996 in “Journal of Investigative Dermatology” 441 citations
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May 1996 in “Journal of Cell Science” Keratin 19 helps identify skin stem cells, with its presence varying by body location, age, and culture stage.
9 citations
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January 1990 in “Skin Pharmacology and Physiology” The method and source of keratinocytes affect the structure of reconstructed skin.
238 citations
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May 1989 in “Journal of Investigative Dermatology”
10 citations
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October 2000 in “PubMed” The study investigated the effects of expressing human papillomavirus type 16 E6/E7 oncogenes in the outer root sheath of hair follicles in transgenic mice. These mice exhibited a fur phenotype with lower hair density but faster hair regeneration compared to wild-type mice. The expression of these oncogenes extended the growth phase (anagen) of hair follicles and bypassed the resting phase (telogen), leading to continuous hair follicle cycling. Although the first hair growth cycle appeared normal, the second cycle showed delayed initiation of catagen and insensitivity to telogen resting signals, even with estradiol present. This indicated that E6/E7 expression in the ORS delayed catagen entry and prevented telogen rest, resulting in ongoing hair follicle cycling.
94 citations
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February 1994 in “The journal of investigative dermatology/Journal of investigative dermatology” EGF makes hair follicles grow longer but stops hair production.
59 citations
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August 1981 in “PubMed” Trichilemmal keratinization is a unique process in hair follicles where the outer root sheath turns into keratin without a specific layer.
