An Improved and Rapid Method to Construct Skin Equivalents from Human Hair Follicles and Fibroblasts

August 2001 in “ Experimental dermatology ”

Daniela Hoeller, Berthold Huppertz, Thomas C. Roos, Pamela Poblete Gutiérrez, Hans F. Merk, Jorge Frank, Frank K. Jugert

skin equivalents human hair follicles fibroblasts collagen dermal equivalent keratinocytes epidermis autologous transplantation skin models hair roots skin cells skin protein skin layer skin surface self-transplantation

TLDR Researchers created a quick, cost-effective way to make skin-like tissue from hair follicles and fibroblasts.

The study presented a rapid, easy, and cost-effective method to construct three-dimensional skin equivalents (SE) using human dermal fibroblasts, collagen, and freshly plucked hair follicles. The process involved polymerizing acidic liquid collagen with sodium hydroxide in the presence of fibroblasts to form a dermal equivalent (DE). Hair follicles were implanted into the DEs, and within 14 days, keratinocytes from the hair follicles formed a fully developed, multi-layered, and cornified epidermis. Histological and immunofluorescence analyses confirmed the similarity of these SEs to normal human skin. The method showed potential for autologous transplantation, reducing the risk of transplant rejection.

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Research cited in this study

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community I started rapidly diffuse thinning at 22. I'm 42 now and have as much hair as I did at 16. AMA

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