Proteomics Characterization of Primary Human Oral Epithelial Cells Using a Novel Culture Technique for Tissue Regeneration
August 2015
in “
PubMed Central
”
Epithelial-derived Pop-Up Keratinocytes ePUKs Gravity Assisted Cell Sorting LC/MS/MS DKK1 SERPINE1 follistatin tenascin-C hair follicle development stem cell niche tissue-engineered oral mucosa EVPOME regenerative medicine wound healing pop-up keratinocytes liquid chromatography-mass spectrometry proteomics stem cells tissue regeneration
TLDR Epithelial-derived Pop-Up Keratinocytes (ePUKs) may enhance wound healing in regenerative medicine.
The study characterized a unique population of oral mucosa keratinocytes called Epithelial-derived Pop-Up Keratinocytes (ePUKs) using a novel culture technique. These undifferentiated cells were isolated using Gravity Assisted Cell Sorting and analyzed through LC/MS/MS, revealing increased expression of proteins like DKK1, SERPINE1, follistatin, and tenascin-C, which are crucial for cellular movement, hair follicle development, and stem cell niche maintenance. The use of ePUKs in fabricating tissue-engineered oral mucosa (EVPOME) demonstrated an increased abundance of these proteins, suggesting that ePUKs could be a valuable cell source for regenerative medicine due to their potential to promote wound healing.
