Mesenchymal Stem Cells From Mouse Hair Follicles Reduce Hypertrophic Scarring in a Murine Wound Healing Model

In a study aimed at improving wound healing and reducing hypertrophic scarring, mesenchymal stem cells (moMSCORS) isolated from the outer root sheath of mouse whisker hair follicles were used in a full-thickness wound model in C57BL/6 mice. These cells were expanded and characterized in vitro, meeting the criteria for mesenchymal stem cells. When applied to wounds, moMSCORS-treated wounds exhibited less inflammation, faster wound closure, and reduced hypertrophic scarring compared to untreated wounds. This was evidenced by clinical scoring, software-based quantification, and histological staining, which showed lower cellularity, fewer collagen filaments, and thinner dermal and epidermal layers in treated wounds. The study demonstrates the potential of moMSCORS in autologous cell therapy for wound healing and offers a viable in vivo model for further research.