Morphogenetic Events in Hair Follicle Heterotypic Spheroids

September 2017 in “ Journal of Investigative Dermatology ”

Ekaterina P. Kalabusheva, E. A. Vorotelyak

hair follicle dermal papilla cells keratinocytes morphogenesis hyaluronic acid CD44 microvillus-type plasma membrane protrusions HF markers self-assembly proliferative cells DPCs KCs HA

TLDR The research concluded that hyaluronic acid affects the formation and growth of hair follicle-like structures in a lab setting.

The study aimed to create human hair follicle (HF) germ-like structures in vitro and investigated the formation of aggregates using dermal papilla cells (DPCs) and keratinocytes (KCs). The researchers found that mixed aggregates of DPCs and KCs showed upregulation of HF markers and proper self-assembly initiation, which was essential for morphogenesis. In contrast, coated aggregates did not exhibit morphogenetic events. The size and number of proliferative cells in mixed aggregates were influenced by the hair-inducing abilities of the DPCs. The structure and morphology of mixed aggregates were characterized, with DPCs forming round-shaped structures and KCs generating multilayered trabeculae. The addition of hyaluronic acid (HA) to the cell suspension before aggregate formation increased the size of aggregates and the number of proliferative cells, but did not maintain DPC-specific markers during 2D cultivation. CD44 staining revealed microvillus-type plasma membrane protrusions on DPCs in mixed aggregates, which are involved in HA metabolism. The study concluded that HA has a significant impact on aggregate formation, self-organization, and HF development.

View this study on jidonline.org →

Discuss this study in the Community →

Related Community Posts Join

6 / 1000+ results

community New and Interesting HairLoss Studies/Papers/Reviews

in Treatment 42 upvotes 4 years ago

Hair loss treatments discussed include Dutasteride with Ketoconazole, tissue engineering strategies, and androgenetic alopecia therapies. Massage doubles follicular retention, improving treatment effectiveness.

community DIY treatment... Shedding completely stopped after 1.5 yrs

in Treatment 245 upvotes 2 years ago

Vitamin C mixed with shampoo stopped shedding and promoted hair regrowth after 1.5 years. Both the person and their wife experienced significant hair loss reduction with this method.

community COMPLETE OVERVIEW of the Treatment of androgenetic alopecia in men

26 upvotes 10 years ago

Male androgenetic alopecia is commonly treated with topical minoxidil and oral finasteride, both requiring continuous use. Other options include hair restoration surgery, dutasteride, light therapy, and camouflaging agents.

community Topical Finasteride Doesn't Directly Reduce 5ar Enzyme on Scalp

in Finasteride/Dutasteride 72 upvotes 1 year ago

Topical Finasteride doesn't directly reduce 5ar enzyme on scalp and has the same mechanism as oral, needing to go through the liver. Users debate the accuracy of this information and discuss various studies and experiences.

community HairClone developing cell replacement treatments for Hair Loss

6 upvotes 5 years ago

HairClone is developing cell replacement treatments to rejuvenate and generate hair follicles, and has launched a crowdfunding campaign. A user expressed skepticism about the need for crowdfunding.

community Diffuse hair loss and scalp biopsies

in Research 692 upvotes 3 months ago

Scalp biopsies are crucial for diagnosing hair loss conditions like Diffuse Unpatterned Alopecia (DUPA) and retrograde hair loss, as treatments like finasteride and dutasteride may not be effective if other conditions are present. Combining PPAR-GAMMA agonists with retinoids could improve treatments for conditions like Lichen Planopilaris.

Related Research

6 / 6 results

research Possible Cellular Communication with Human Follicle Dermal Papilla Cells via Secretions from Human Hair Follicle Keratinocytes

January 2022 in “Journal of St. Marianna University”

Substances from human hair cells can affect hair loss-related genes, potentially leading to new treatments for baldness.

research A Model System to Analyze the Ability of Human Keratinocytes to Form Hair Follicles

15 citations, April 2014 in “Experimental Dermatology”

Scientists developed a system to study human hair growth using skin cells, which could help understand hair development and improve skin substitutes for medical use.

research Review of Hair Follicle Dermal Cells

321 citations, December 2009 in “Journal of Dermatological Science”

Dermal cells are key in controlling hair growth and could potentially be used in hair loss treatments, but more research is needed to improve hair regeneration methods.