MmuPV1-Induced Cutaneous Squamous Cell Carcinoma Arises Preferentially from Lgr5+ Epithelial Progenitor Cells

August 2022 in “ Viruses ”

Ruben Moreno, Darya Buehler, Paul F. Lambert

cutaneous squamous cell carcinoma Lgr5+ epithelial progenitor cells murine papillomavirus lineage tracing Lgr5-eGFP-IRES-CreERT2 Rosa26LSLtdTomato tdTomato+ cells squamous dysplasia Sox9 phospho-S6 cSCC Lgr5+ cells MmuPV1 Lgr5-eGFP Rosa26 tdTomato

TLDR Skin cancer often starts from Lgr5+ progenitor cells.

The study investigates the role of Lgr5+ epithelial progenitor cells in the development of cutaneous squamous cell carcinoma (cSCC) induced by murine papillomavirus (MmuPV1) in mice. Using lineage tracing with Lgr5-eGFP-IRES-CreERT2/Rosa26LSLtdTomato mice, the researchers found that cSCC lesions had a significantly higher percentage of tdTomato+ cells (42%) compared to squamous dysplasia (7%), indicating that cSCCs preferentially arise from Lgr5+ progenitor cells. This suggests that Lgr5+ cells play a crucial role in the pathogenesis of MmuPV1-induced cSCC. Additionally, increased Sox9 and phospho-S6 expression were observed in cSCC, further characterizing the molecular environment of these tumors.

View this study on mdpi.com →

Discuss this study in the Community →

Cited in this study

7 / 7 results

research NF-κB Participates in Mouse Hair Cycle Control and Plays Distinct Roles in Various Pelage Hair Follicle Types

23 citations , February 2018 in “The journal of investigative dermatology/Journal of investigative dermatology”

NF-κB is crucial for different stages and types of hair growth in mice.

research Quiescent Tissue Stem Cells Evade Immune Surveillance

169 citations , February 2018 in “Immunity”

Inactive stem cells in hair follicles and muscles can avoid detection by the immune system.

research The HPV16 Oncogenes Cause Aberrant Stem Cell Mobilization

23 citations , September 2013 in “Virology”

HPV16 oncogenes disrupt the normal activity of hair follicle stem cells.

research Epithelial Stem Cells And Implications For Wound Repair

156 citations , December 2012 in “Seminars in Cell & Developmental Biology”

Different types of stem cells in hair follicles play unique roles in wound healing and hair growth, with some stem cells not originating from existing hair follicles but from non-hair follicle cells. WNT signaling and the Lhx2 factor are key in creating new hair follicles.

research The Hair Follicle as a Dynamic Mini-Organ

759 citations , February 2009 in “Current Biology”

Hair follicles are complex, dynamic mini-organs that help us understand cell growth, death, migration, and differentiation, as well as tissue regeneration and tumor biology.

research Lgr5 Marks Cycling, Yet Long-Lived, Hair Follicle Stem Cells

835 citations , October 2008 in “Nature Genetics”

Lgr5 is a marker for active, long-lasting stem cells in mouse hair follicles.

research Morphogenesis and Renewal of Hair Follicles from Adult Multipotent Stem Cells

949 citations , January 2001 in “Cell”

Adult mouse skin contains stem cells that can create new hair, skin, and oil glands.