An Isotope Dilution-Liquid Chromatography-Tandem Mass Spectrometry-Based Candidate Reference Measurement Procedure for the Quantification of 5-Alpha-Dihydrotestosterone in Human Serum and Plasma

January 2026 in “ Clinical Chemistry and Laboratory Medicine (CCLM) ”

L.L. Lewin, Michael Stadlmeier, Neeraj Singh, Friederike Bauland, Daniel Köppl, Alexander Gaudl, Andrea Geistanger, Uta Ceglarek, Manfred Rauh, Judith Taibon

5-alpha-dihydrotestosterone DHT 5α-DHT 5β-DHT isotope dilution-liquid chromatography-tandem mass spectrometry dihydrotestosterone

TLDR The procedure accurately measures 5-alpha-dihydrotestosterone in blood, useful for standard tests.

The study presents a reference measurement procedure (RMP) using isotope dilution-liquid chromatography-tandem mass spectrometry for quantifying 5-alpha-dihydrotestosterone (DHT) in human serum and plasma. The RMP demonstrated high analytical performance and met the necessary requirements for measurement uncertainty. It effectively differentiated between the 5α-DHT and 5β-DHT isomers, making it suitable for routine assay standardization and clinical sample evaluation.

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An Isotope Dilution-Liquid Chromatography-Tandem Mass Spectrometry-Based Candidate Reference Measurement Procedure for the Quantification of 5-Alpha-Dihydrotestosterone in Human Serum and Plasma

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