High-Throughput and Direct Measurement of Androgen Levels Using Turbulent Flow Chromatography Liquid Chromatography-Triple Quadrupole Mass Spectrometry to Discover Chemicals That Modulate Dihydrotestosterone Production in Human Prostate Cancer Cells

    November 2017 in “ Biotechnology Letters ”
    Kyungsu Kang, Liming Peng, Yu-Jin Jung … Yong-Soo Choi
    Studysummary This study developed a high-throughput method that identifies bioactive chemicals influencing DHT production in prostate cancer cells, with potential nutraceutical candidates including fucoxanthin, phenethyl caffeate, and Curcuma longa L. extract.
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    Research cited in this study 6

    1. Simultaneous Quantitation of Nine Hydroxy-Androgens and Their Conjugates in Human Serum by Stable Isotope Dilution Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry Journal of steroid biochemistry and molecular biology/˜The œJournal of steroid biochemistry and molecular biology · 2017
    2. A New Label-Free Screen for Steroid 5α-Reductase Inhibitors Using LC-MS Steroids · 2016
    3. 5α-Reductase Inhibitors in Androgenetic Alopecia Current Opinion in Endocrinology, Diabetes and Obesity · 2014
    4. Simultaneous Pharmacokinetic and Pharmacodynamic Analysis of 5α-Reductase Inhibitors and Androgens by Liquid Chromatography Tandem Mass Spectrometry Talanta · 2014
    5. Anti-Androgenic Effect of Sesquiterpenes Isolated from the Rhizomes of Curcuma Aeruginosa Roxb. Fitoterapia · 2012
    6. The 5 Alpha-Reductase Isozyme Family: A Review of Basic Biology and Their Role in Human Diseases Advances in Urology · 2011