High-Throughput and Direct Measurement of Androgen Levels Using Turbulent Flow Chromatography Liquid Chromatography-Triple Quadrupole Mass Spectrometry to Discover Chemicals That Modulate Dihydrotestosterone Production in Human Prostate Cancer Cells

November 2017 in “ Biotechnology Letters ”

Kyungsu Kang, Liming Peng, Yu-Jin Jung, Joo Yeon Kim, Eun Ha Lee, Hee Ju Lee, Sang Min Kim, Sang Hyun Sung, Cheol-Ho Pan, Yong-Soo Choi

androgen levels turbulent flow chromatography liquid chromatography triple quadrupole mass spectrometry TFC-LC-TQMS testosterone dihydrotestosterone DHT prostate cancer cells phytochemicals fucoxanthin phenethyl caffeate Curcuma longa L. extract naringenin androgen-modulatory nutraceuticals Curcuma longa L. turmeric

TLDR Researchers found four natural compounds that can change DHT levels in prostate cancer cells.

In 2017, researchers used a high-throughput method called turbulent flow chromatography liquid chromatography-triple quadrupole mass spectrometry (TFC-LC-TQMS) to measure the conversion of testosterone to dihydrotestosterone (DHT) in human prostate cancer cells. The method screened 103 samples in less than 12 hours. The study identified four phytochemicals that affected DHT production: fucoxanthin, phenethyl caffeate, Curcuma longa L. extract, and naringenin. Fucoxanthin, phenethyl caffeate, and Curcuma longa L. extract inhibited DHT production, while naringenin increased it. These phytochemicals could potentially be used to adjust DHT levels in male hormonal dysfunction, but further studies are needed to develop them as androgen-modulatory nutraceuticals.

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