Simultaneous Quantitation of Nine Hydroxy-Androgens and Their Conjugates in Human Serum by Stable Isotope Dilution Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry

    January 2017 in “ Journal of steroid biochemistry and molecular biology/˜The œJournal of steroid biochemistry and molecular biology ”
    Tianzhu Zang, Daniel Tamae, Clementina Mesaros, Qingqing Wang, Manna Huang, Ian A. Blair, T.M. Penning
    TLDR A new method accurately measures nine specific hormones in human blood.
    The study developed and validated a stable isotope dilution liquid chromatography electrospray ionization tandem mass spectrometry (SID-LC-ESI–MS/MS) method for the simultaneous quantitation of nine hydroxy-androgens and their conjugates in human serum. This method demonstrated high sensitivity, specificity, and reproducibility, meeting FDA criteria for bioanalytical method validation. It revealed significant gender differences in androgen levels and highlighted the importance of enzymatic hydrolysis for accurate quantitation. The method's reliability and precision make it a valuable tool for clinical and research applications in endocrinology, particularly for investigating androgen biosynthesis, drug resistance mechanisms in castration-resistant prostate cancer (CRPC), and androgen-related conditions.
    Discuss this study in the Community →

    Research cited in this study

    3 / 3 results

    Related Community Posts Join

    6 / 11 results

      community The theory that explains everything. Please help me make this big!

      in Research/Science  2450 upvotes 4 years ago
      The post and conversation are about the role of the enzyme 3alpha-hydroxysteroid reductase in hair loss and the potential of compounds like procyanidin B2 and sulforaphane to boost its activity for hair regrowth. Further research is needed to develop effective treatments based on this theory.

      community Interpreting pre-finisteride blood panels

      in Chat  2 upvotes 1 month ago
      The conversation is about someone planning to start finasteride for hair loss and wanting to interpret pre-treatment blood panels to assess the risk of side effects like gynecomastia. They list various tests to measure hormone levels and other health indicators.

      community Pre-Finisteride DHT & Hormone Blood Panel

      in Finasteride/Dutasteride  3 upvotes 1 month ago
      The conversation discusses the cost and insurance coverage of a comprehensive hormone and DHT blood panel for hair loss, with a focus on finasteride treatment. The user seeks a cheaper alternative to a $700 panel recommended in a video.

      community Dutasteride 1mg or RU58841 8% or topical Dutasteride

      in Treatment  3 upvotes 1 month ago
      The discussion focuses on hair loss treatments, with suggestions to increase oral Dutasteride to 1 mg or more, as topical Dutasteride is less effective. Other treatments mentioned include RU58841, Minoxidil, and dermastamping, with some users recommending injectable mesotherapy and topical Finasteride.

    Similar Research

    5 / 1000+ results