Simultaneous Quantitation of Nine Hydroxy-Androgens and Their Conjugates in Human Serum by Stable Isotope Dilution Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry

    January 2017 in “ Journal of steroid biochemistry and molecular biology/˜The œJournal of steroid biochemistry and molecular biology ”
    Tianzhu Zang, Daniel Tamae, Clementina Mesaros, Qingqing Wang, Manna Huang, Ian A. Blair, T.M. Penning
    TLDR A new method accurately measures nine specific hormones in human blood.
    The study developed and validated a stable isotope dilution liquid chromatography electrospray ionization tandem mass spectrometry (SID-LC-ESI–MS/MS) method for the simultaneous quantitation of nine hydroxy-androgens and their conjugates in human serum. This method demonstrated high sensitivity, specificity, and reproducibility, meeting FDA criteria for bioanalytical method validation. It revealed significant gender differences in androgen levels and highlighted the importance of enzymatic hydrolysis for accurate quantitation. The method's reliability and precision make it a valuable tool for clinical and research applications in endocrinology, particularly for investigating androgen biosynthesis, drug resistance mechanisms in castration-resistant prostate cancer (CRPC), and androgen-related conditions.
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