Immunolocalization of a Histidine-Rich Epidermal Differentiation Protein in the Chicken Supports the Hypothesis of an Evolutionary Developmental Link Between the Embryonic Subperiderm and Feather Barbs and Barbules

The study investigated the localization of a histidine-rich epidermal differentiation protein, EDMTFH, in chicken embryos to explore its role in feather development. Using immunohistochemical and immunogold labeling techniques, researchers found that EDMTFH was present in the subperiderm and in the barbs and barbules of feathers, but not in other epidermal layers or the feather sheath. This protein's distribution pattern suggested it contributes to the mechanical properties of feathers and supports the hypothesis of an evolutionary developmental link between the embryonic subperiderm and feather structures.