Expansion of Hair Follicle Stem Cells Sticking to Isolated Sebaceous Glands to Generate In Vivo Epidermal Structures

June 2016 in “ Cell Transplantation ”

Huishan Zhang, Huashan Zhao, Jingqiao Qiao, Shoubing Zhang, Shuang Liu, Na Li, Xiaohua Lei, Lina Ning, Yuqi Cao, Enkui Duan

hair follicle stem cells sebaceous glands keratinocytes basal layers bulge stem cells upper isthmus stem cells epidermis hair follicles skin reconstitution assay HFSCs SGs

TLDR Sebaceous glands can help harvest hair follicle stem cells to regenerate skin and hair.

The study developed a novel method to efficiently isolate and expand hair follicle stem cells (HFSCs) from small skin biopsies by capturing sebaceous glands (SGs) and their adjacent HFSCs. These cells, expressing key stem cell markers, were capable of reconstituting new epidermis, hair follicles, and SGs in vivo. Tested on rat tail skin and validated in nude mice, this technique provided a high-output method for obtaining HFSCs without needing large skin samples or intact hair follicles, enhancing their potential for clinical applications in wound repair and skin tissue engineering.

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