Defining the identity of mouse embryonic dermal fibroblasts

    June 2016 in “Genesis
    Isadore Budnick, Emily Hamburg-Shields, Demeng Chen, Enrique Torre, Andrew Jarrell, Batool Akhtar-Zaidi, Olivia Cordovan, Rob C. Spitale, Peter C. Scacheri, Radhika P. Atit
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    TLDR Researchers identified specific genes that are important for mouse skin cell development and healing.
    In 2016, researchers conducted a study to define the gene expression signature of mouse embryonic dermal fibroblasts, which are crucial for hair follicle development and scarless wound healing. They used Shannon entropy analysis on microarray data and identified 63 genes with enriched expression in these fibroblasts. The study revealed that a subset of these genes was dependent on Wnt/β-catenin signaling, important for fibroblast fate and hair follicle induction. The signature was validated through RNA-sequencing and other techniques. Additionally, the study examined histone modifications and DNase I hypersensitivity, suggesting a lineage similarity between certain mesoderm cells and dermal fibroblasts. Transcription factors like Klf7 and Zfp281 were implicated in regulating the signature. The research also linked the gene expression signature to diseases by analyzing GWAS data, finding associations with lung disease, electrical heart function, and skin defects. The findings provide a deeper understanding of embryonic dermal fibroblast identity and their role in development and disease.
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