Aligned membranes regulate wound healing via MMP12 secreted by macrophages

The study explores the impact of aligned electrospinning membranes on wound healing, emphasizing the role of MMP12 secreted by macrophages. Through in vivo and in vitro experiments using a mouse model, the research shows that these membranes enhance wound healing by upregulating MMP12, which inhibits fibroblast migration and reduces scar formation. The aligned membranes create a regenerative microenvironment that promotes efficient, scar-free healing, with increased hair follicle regeneration and organized collagen deposition. Single-cell RNA sequencing identified MMP12 overexpression in a macrophage subcluster, enhancing phagocytosis and modulating immune responses. The study suggests that these membranes could be used to develop advanced biological dressings for improved skin repair outcomes.