Establishment of an Immortalized Mouse Dermal Papilla Cell Strain with Optimized Culture Strategy

January 2018 in “ PeerJ ”

Haiying Guo, Yizhan Xing, Yiming Zhang, Long He, Fang Deng, Xiaogen Ma, Yuhong Li

dermal papilla cells hair growth hair regeneration collagen I α-MEM SV40 large T antigen immortalized cells hair follicle development hair loss treatments DP cells collagen alpha-MEM SV40

Studysummary This study successfully optimized a culture strategy for dermal papilla cells and established an immortalized cell strain, iDP6, which is suitable for research and application.

Our plain-language summary. Not medical advice or a treatment recommendation. Consult a qualified healthcare professional before changing treatment. Full disclaimer

In 2018, researchers developed an optimized culture strategy for mouse dermal papilla (DP) cells, which are crucial for hair growth and regeneration. They used a stepwise dissection, collagen I coated plate, and α-MEM based culture medium. The team also immortalized the primary DP cells using SV40 large T antigen, a protein that extends cell lifespan. They established several cell strains, including iDP6, which was similar to primary DP cells. The study also found that the immortalization process was reversible. This research could be beneficial for future studies on hair follicle development and potential hair loss treatments.

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Research cited in this study

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research Methods for the Isolation and 3D Culture of Dermal Papilla Cells from Human Hair Follicles

55 citations , April 2017 in “Experimental Dermatology”

This article details laboratory methods for isolating and culturing human dermal papilla cells but provides no new experimental results.

research Epithelial-Mesenchymal Micro-Niches Govern Stem Cell Lineage Choices

214 citations , April 2017 in “Cell”

This study found that micro-niches within hair follicles create heterogeneity among stem cells and transit-amplifying cells, leading to specialized progenitors that control tissue morphogenesis and regeneration.

research Effect of Sinapic Acid on Hair Growth Promotion in Human Hair Follicle Dermal Papilla Cells via Akt Activation

25 citations , March 2017 in “Archives of Dermatological Research”

This study suggests that sinapic acid may promote hair growth in human hair follicle dermal papilla cells by activating specific signaling pathways and inducing the expression of growth factors.

research Icariin Promotes Mouse Hair Follicle Growth by Increasing Insulin-Like Growth Factor 1 Expression in Dermal Papillary Cells

22 citations , February 2017 in “Clinical and Experimental Dermatology”

This study suggests that icariin promotes hair follicle growth in mice by enhancing IGF-1 expression in dermal papilla cells.

research Wnt/β-Catenin Signaling Pathway Activates Melanocyte Stem Cells In Vitro and In Vivo

45 citations , April 2016 in “Journal of Dermatological Science”

This study found that the Wnt/β-catenin signaling pathway activates melanocyte stem cells, promoting hair follicle regeneration both in vitro and in vivo.

Surface Tension Guided Hanging-Drop: Producing Controllable 3D Spheroids of High-Passaged Human Dermal Papilla Cells and Forming Inductive Microtissues for Hair-Follicle Regeneration

research Surface Tension Guided Hanging-Drop: Producing Controllable 3D Spheroids of High-Passaged Human Dermal Papilla Cells and Forming Inductive Microtissues for Hair-Follicle Regeneration

62 citations , February 2016 in “ACS Applied Materials & Interfaces”

This study found that 3D microtissue models of dermal papilla cells can enhance the ability to induce hair-follicle neogenesis in vivo, offering potential for controlled cell production in follicle regeneration.

research Effects of Dihydrotestosterone on Rat Dermal Papilla Cells In Vitro

19 citations , April 2015 in “European Journal of Pharmacology”

This study found that dihydrotestosterone (DHT) may shorten the hair growth cycle through mechanisms like cell-cycle arrest and β-catenin downregulation in rat hair follicle cells.

research Paracrine Crosstalk Between Human Hair Follicle Dermal Papilla Cells and Microvascular Endothelial Cells

35 citations , February 2015 in “Experimental Dermatology”

This study found that human follicle dermal papilla cells promote survival, proliferation, and tubulogenesis of microvascular endothelial cells, highlighting their significant role in hair follicle vascular remodeling.

research Hair Follicle Dermal Stem Cells Regenerate the Dermal Sheath, Repopulate the Dermal Papilla, and Modulate Hair Type

184 citations , November 2014 in “Developmental Cell”

This study identifies a bipotent stem cell in adult hair follicles that self-renews and contributes to dermal sheath and papilla cell populations, potentially aiding hair regrowth after injury, disease, or aging.

The Dermal Papilla: An Instructive Niche for Epithelial Stem and Progenitor Cells in Development and Regeneration of the Hair Follicle

research The Dermal Papilla: An Instructive Niche for Epithelial Stem and Progenitor Cells in Development and Regeneration of the Hair Follicle

149 citations , July 2014 in “Cold Spring Harbor Perspectives in Medicine”

This article provides contact information for Bruce A. Morgan and features no new research findings.

research Multipotent Neural Crest Stem Cell-Like Cells from Rat Vibrissa Dermal Papilla Induce Neuronal Differentiation of PC12 Cells

10 citations , January 2014 in “BioMed research international”

In this study, dermal papilla cells from rat vibrissae showed potential for treating CNS injuries by secreting neurotrophic factors and promoting neuronal differentiation more effectively than bone marrow mesenchymal stem cells.

research A Review of Adipocyte Lineage Cells and Dermal Papilla Cells in Hair Follicle Regeneration

35 citations , January 2014 in “Journal of Tissue Engineering”

This review discusses recent advances in the role of dermal papilla cells and adipocyte lineage cells in hair regeneration, but it reports no new clinical results.

Microenvironmental Reprogramming by Three-Dimensional Culture Enables Dermal Papilla Cells to Induce De Novo Human Hair-Follicle Growth

research Microenvironmental Reprogramming by Three-Dimensional Culture Enables Dermal Papilla Cells to Induce De Novo Human Hair-Follicle Growth

256 citations , October 2013 in “Proceedings of the National Academy of Sciences of the United States of America”

This study found that altering cell culture conditions to form three-dimensional papilla spheroids can restore the ability of human dermal papilla cells to induce hair growth in adult skin.

Dissociated Human Dermal Papilla Cells Induce Hair Follicle Neogenesis in Grafted Dermal-Epidermal Composites

research The Establishment and Characterization of Immortalized Human Dermal Papilla Cells and Their Hair Growth Promoting Effects

13 citations , September 2010 in “Journal of Dermatological Science”

Researchers made a cell line that grows quickly and can help with hair growth research.