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This study found that suspension culture was more effective than hydrogel-based systems for maintaining spheroid morphology and differentiation of human iPSC-derived definitive endoderm cells.

This study found that using three-dimensional cell culture with Matrigel enhances the biological function and stemness of stem cells, leading to improved soft tissue repair and healing by activating the host microenvironment and autologous stem cells.

This study reported the successful isolation of epithelial stem cells from mouse embryonic skin using a simple culture method, with the cells showing the potential to differentiate into multiple cell types.

This study explored a cell-free therapy with mesenchymal stem cell paracrine proteins and PEG hydrogels for deep burn treatment and found that the combination significantly enhanced wound healing, reduced scar formation, and promoted skin appendage regeneration in a rabbit model.

This study found that a novel 3D in vitro culture condition improved stemness markers and proliferation potential in human dermal stem/progenitor cells compared to traditional 2D methods.