Tissue-engineered dermal substitutes constructed by human embryonic stem cell-derived fibroblasts facilitate the repair of skin wounds

This study demonstrates that human embryonic stem cell-derived fibroblasts (hESC-Fbs) can be effectively used to construct tissue-engineered dermal substitutes for skin wound repair. By directly inducing hESCs to differentiate into mesenchymal stem cells and then into fibroblasts without the embryoid body stage, researchers achieved high efficiency and purity. The hESC-Fbs expressed growth factors and extracellular matrix components similar to human skin fibroblasts, and successfully repaired mouse skin wounds by day 20 post-transplantation. This EB-free induction strategy shows promise for clinical applications in treating large-area skin wounds.