Updated Skin Transcriptomic Atlas Depicted by Reciprocal Contribution of Single-Nucleus RNA Sequencing and Single-Cell RNA Sequencing

The document presents a study that used both single-cell RNA sequencing (scRNA-seq) and single-nucleus RNA sequencing (snRNA-seq) to profile skin cells. The study found that snRNA-seq identified more relevant keratinocyte clusters, which are important for skin cell differentiation. New markers for different stages of keratinocyte differentiation were discovered, including LYPD3, EMP2, and CSTB. Additionally, transcription factors NFIB and GRHL1 were identified as being involved in keratinocyte proliferation and differentiation. The study also found that scRNA-seq detected a greater number of immune cells, particularly activated ones. Fibroblasts were found to be the core of cell-to-cell interactions, and ligands of the COLLAGEN, LAMININ, and SEMA3 signaling pathways were enriched in fibroblasts in snRNA-seq, indicating their crucial role in maintaining skin homeostasis. The study thus provides an updated atlas of the skin transcriptome.