Updated Skin Transcriptomic Atlas Depicted by Reciprocal Contribution of Single-Nucleus RNA Sequencing and Single-Cell RNA Sequencing

April 2023 in “ Journal of Investigative Dermatology ”

R. Zhu, X. Pan, S. Wang, Q. Qiong, C. Gu, X. Yao, W. Li

New to collagen? There is a guide in the encyclopedia. Read the guide →

single-cell RNA sequencing single-nucleus RNA sequencing keratinocyte keratinocyte differentiation LYPD3 EMP2 CSTB NFIB GRHL1 immune cells fibroblasts COLLAGEN LAMININ SEMA3 skin homeostasis scRNA-seq snRNA-seq

Studysummary This study found that single-nucleus RNA sequencing identified more relevant keratinocyte clusters and specific markers than single-cell RNA sequencing, offering a new perspective on skin cell differentiation and function.

Our plain-language summary. Not medical advice or a treatment recommendation. Consult a qualified healthcare professional before changing treatment. Full disclaimer ·

View this study on jidonline.org →

Discuss this study in the Community →

Similar Research

5 / 1000+ results

research Mapping Human Skin: One Sequenced Cell at a Time

November 2023 in “The journal of investigative dermatology/Journal of investigative dermatology”

This study highlighted the significance of integrating single-cell RNA sequencing with spatial transcriptomics for improving cell-type identification in human skin, emphasizing the need for a comprehensive cell atlas.

research Efficient integration of human skin single-cell RNA sequencing data

December 2025

The researchers developed a comprehensive human skin cell atlas using data from various studies and established a consensus nomenclature for normal human skin in this project, which also includes a deep learning-based method for more effective reference mapping of new cells.

Single Cell Transcriptome Profiling of Human Interfollicular Epidermis Reveals Robust Heterogeneity and Divergent Differentiation Lineages

research 1337 Single cell transcriptome profiling of human interfollicular epidermis reveals robust heterogeneity and divergent differentiation lineages

April 2018 in “Journal of Investigative Dermatology”

This study used single-cell RNA sequencing to identify at least nine subpopulations of keratinocytes in human neonatal epidermis, revealing unexpected heterogeneity and complex differentiation trajectories.

research Revisiting the significance of keratin expression in complex epithelia

6 citations , October 2022 in “Journal of cell science”

This study re-analyzed single-cell RNAseq data from human and mouse skin, confirming and refining insights into keratin gene regulation during keratinocyte differentiation in epithelial tissues.

research Efficient integration of human skin single-cell RNA sequencing data

December 2025

This research by Yuan et al. focused on developing a comprehensive human skin cell atlas, analyzing various cell types and diseases, and introduced a deep learning method, scSEA, for unbiased reference mapping, potentially discovering new cell types.