The Proximal Promoter of the Human Transglutaminase 3 Gene

February 1996 in “ Journal of biological chemistry/The Journal of biological chemistry ”

Jeung‐Hoon Lee, Shyh‐Ing Jang, Jun‐Mo Yang, Nedialka G. Markova, Peter M. Steinert

transglutaminase 3 TGM3 epidermal differentiation hair follicle cell function epidermal keratinocytes Sp1 ets-like recognition motifs gene therapy promoter/enhancer activity

Studysummary In this study, sequences upstream of the TGM3 gene were found to regulate epithelial-specific gene expression in keratinocytes, suggesting potential applications in gene therapy.

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The study investigated the regulation of the transglutaminase 3 gene (TGM3), which is important for the late stages of epidermal differentiation and hair follicle cell function. Researchers analyzed 3.0 kilobase pairs upstream of the TGM3 transcription start site and identified the proximal promoter region between −126 and −91. This region was found to confer epithelial-specific expression in epidermal keratinocytes. The study revealed that the interaction between Sp1- and ets-like recognition motifs is crucial for TGM3 promoter function. The findings suggested that these sequences could be useful for gene therapy in keratinocytes due to their strong promoter/enhancer activity.

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