Studysummary In this study, researchers identified a highly reactive, isozyme-specific sequence for TGase 3, contributing to understanding its distinct functional role and activity distribution in the mouse epidermis. Our plain-language summary of this paper — not a Tressless recommendation.
The study identified a preferred substrate peptide, E51 (PPPYSFYQSRWV), for transglutaminase 3 (TGase 3) using a phage-displayed peptide library, demonstrating high reactivity and specificity with minimal cross-reactivity to other isozymes. Alanine substitution analysis highlighted crucial amino acids for substrate recognition. The peptide form, pepE51, confirmed its potential as a probe for detecting TGase 3 activity in situ, revealing its presence in specific layers of the epidermis and hair follicles. This research provided a valuable tool for studying TGase 3 activity and its role in skin and hair follicle physiology, aiding in the investigation of related diseases.
30 citations
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August 2008 in “The journal of investigative dermatology/Journal of investigative dermatology” This study suggests that TGase 3 may contribute to hair fiber scaffolding by forming specific isopeptide bonds between keratin intermediate filaments and keratin-associated proteins.
100 citations
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March 2006 in “Journal of biological chemistry/The Journal of biological chemistry” This study found that cystatin M/E strongly inhibits human cathepsin V and cathepsin L via distinct non-overlapping sites, suggesting an important role in human epidermal differentiation and hair follicle morphogenesis.
118 citations
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January 2004 in “European Journal of Cell Biology” Balanced protease activity is crucial for healthy skin and hair development.
425 citations
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August 2002 in “BioEssays” This review discusses the structure and assembly of the cornified cell envelope in stratified squamous epithelial cells and various disorders leading to barrier defects, but reports no new findings.
135 citations
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October 1997 in “Journal of biological chemistry/The Journal of biological chemistry” This study suggests that trichohyalin is modified by peptidyl-arginine deiminase before being cross-linked by TGase 3, allowing the formation of rigid structures in hair follicle cells.
118 citations
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June 1993 in “Journal of Biological Chemistry” This study found that mouse and human protransglutaminase 3 enzymes require calcium-regulated activation for their role in later stages of cell envelope formation in the epidermis and hair follicle.
