Mouse Epidermal Neural Crest Stem Cell Cultures

    May 2008 in “ Journal of Visualized Experiments ”
    Maya Sieber‐Blum, Yaofei Hu
    TLDR Mouse Epidermal Neural Crest Stem Cells can become various cell types.
    The document described the process of isolating and culturing Mouse Epidermal Neural Crest Stem Cells (EPI-NCSC) from the bulge of hair follicles, specifically from whisker follicles of adult mice. EPI-NCSC were identified as multipotent stem cells capable of differentiating into various cell types, such as neurons, nerve supporting cells, smooth muscle cells, bone/cartilage cells, and melanocytes. The protocol involved removing the whisker pad, dissecting the follicles, and isolating the bulge region, from which EPI-NCSC would emigrate after 3 to 4 days in culture.
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