Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures
May 2008
in “
Journal of Visualized Experiments
”
TLDR Mouse epidermal neural crest stem cells can become various cell types and are easily obtained from hair follicles.
Mouse Epidermal Neural Crest Stem Cells (EPI-NCSC) were multipotent stem cells located in the bulge of hair follicles, capable of differentiating into various cell types such as neurons, nerve supporting cells, smooth muscle cells, bone/cartilage cells, and melanocytes. These cells were easily accessible from the hairy skin and could be isolated as a pure population. The document detailed a protocol for preparing EPI-NCSC cultures from mouse whisker follicles, involving the removal and dissection of whisker follicles, followed by the extraction of the bulge region for culture. EPI-NCSC began to emigrate from the bulge explants within 3 to 4 days.