Subcellular Localization of the Five Members of the Human Steroid 5α-Reductase Family

    June 2017 in “ Biochimie open ”
    Antonella Scaglione, Linda Celeste Montemiglio, Giacomo Parisi, Italia Anna Asteriti, Renato Bruni, Gabriele Cerutti, Claudia Testi, C. Savino, Filippo Mancia, Patrizia Lavia, B. Vallone
    TLDR All five human steroid 5α-reductase enzymes are found in the endoplasmic reticulum.
    The study investigated the subcellular localization of the five members of the human steroid 5α-reductase (SRD5A) family using eGFP-tagged proteins in HeLa cells. It found that SRD5A1, SRD5A2, and SRD5A3 predominantly localized in the endoplasmic reticulum (ER), while TECR and TECRL also showed ER localization, except for C-term eGFP-tagged TECR, which formed aggregates. The position of the eGFP tag significantly affected protein expression and localization, with C-term tagging often leading to protein misfolding and aggregation. These findings clarified the ER localization of SRD5A family members and emphasized the need for validation in different cell lines and experimental settings.
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