Isolation and Characterization of Cutaneous Epithelial Stem Cells
January 2013
in “
Humana Press eBooks
”
TLDR Scientists found ways to identify and collect skin stem cells, which vary by skin area and are delicate.
The document from 2013 details the methods for isolating and characterizing cutaneous epithelial stem cells from adult mammalian skin, focusing on the heterogeneity and regionalization of progenitor populations. It describes the use of mouse genetics and antibodies to label and isolate progenitor cells from different skin regions, and outlines clonogenic assays and skin and hair reconstitution assays to assess their regenerative potential. The document also discusses the challenges in isolating specific progenitor cell subsets due to their reduced frequency and the fragility of keratinocytes, recommending adjustments in flow cytometry techniques to prevent issues during cell sorting. The research was supported by an NIH grant and is part of a broader context of research on skin stem cells and their niches.
