In Vitro Expansion of Human Mesenchymal Stem Cells and Hepatocytes Using Purified Platelet-Derived Growth Factors: A Potential Therapeutic Modality for Liver Fibrosis

April 2023 in “ Egyptian journal of Immunology ”

Hanaa Elsaied, Mohamed Elzallat, Aisha Yassin, Shahira Elfedawy, Dina Soliman, Dina Ragab, Ragab Fahmy

platelet-derived growth factors human platelet lysate fetal bovine serum mesenchymal stem cells hepatocytes xenofree culture medium L-GF hPL FBS MSCs

TLDR 10% hPL is best for growing mesenchymal stem cells, while 10% FBS is best for hepatocytes.

This study evaluated the use of purified platelet-derived growth factors (L-GF) and human platelet lysate (hPL) as alternatives to fetal bovine serum (FBS) for culturing mesenchymal stem cells (MSCs) and hepatocytes. The results indicated that 10% hPL was most effective for MSCs, providing the highest cell count, viability, and shortest time to confluency, suggesting its potential as a xenofree culture medium for clinical applications. Conversely, 10% FBS was superior for hepatocyte culture, achieving better cell count, viability, and faster confluency compared to hPL and L-GF.

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