An Immunohistochemical Study on Cell Differentiation in the Outer Root Sheath of Normal Human Anagen Hair Follicles with Antikeratin Monoclonal Antibodies

December 1990 in “ PubMed ”

Masahito Asada, Ichiro Kurokawa, S. Nishijima, Yoshimasa Asada

outer root sheath anagen hair follicles cytokeratins antikeratin monoclonal antibodies CK 1 CK 10 CK 11 keratinocytes infundibulum isthmus trichilemmal keratinization CK 19 stem cells CK 16 hyperproliferative keratin ORS hair canal hair axis

TLDR Keratinocytes in hair follicles differentiate similarly to skin cells, with specific patterns in different regions.

The study investigated the expression of various cytokeratins (CKs) in the outer root sheath (ORS) of human anagen hair follicles using 11 antikeratin monoclonal antibodies on 10 scalp specimens. CKs 1, 10, and 11 were found in intermediate and granular cells at the infundibulum, indicating differentiation similar to interfollicular epidermal keratinocytes. A clear demarcation in CK expression was observed between the infundibulum and isthmus. CK 19, a marker for undifferentiated stem cells, was present in the outermost cells of the ORS at the isthmus and lower ORS. CK 16, associated with hyperproliferative keratin, was detected in the outermost cells of the infundibulum and all ORS cells below the isthmus. The study suggested that ORS cells below the isthmus move upward along the hair axis with differentiation.

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