Simple and Selective HPLC-UV/Vis Bioanalytical Method to Determine Aluminum Phthalocyanine Chloride in Skin Permeation Studies

The study developed a simple and selective HPLC-UV/Vis bioanalytical method to determine aluminum phthalocyanine chloride (AlPcCl) in skin permeation studies, crucial for improving its topical delivery in photodynamic therapy for skin cancers. The method addressed AlPcCl's hydrophobicity and aggregation issues, allowing its quantification in complex skin matrices. Validated for selectivity, linearity, precision, accuracy, and robustness, it effectively distinguished AlPcCl from skin contaminants. In vitro experiments with porcine skin showed high sensitivity and good linearity, with LOD and LOQ of 0.03 and 0.09 μg/mL, respectively. The method was suitable for pharmaceutical formulation assays and skin permeation kinetic studies, supporting the development of dermatological systems to enhance AlPcCl's bioavailability and therapeutic efficacy.