Chromatographic Method for Clobetasol Propionate Determination in Hair Follicles and Different Skin Layers

    August 2016 in “ Biomedical Chromatography ”
    Tamara Ângelo, Marcílio Cunha‐Filho, Guilherme M. Gelfuso, Taís Gratieri
    TLDR A new method accurately measures clobetasol propionate in hair and skin.
    The study developed a simple HPLC-UV method for quantifying clobetasol propionate (CLO) in hair follicles and different skin layers, addressing the need for precise drug delivery in alopecia treatment. Using a RP-C18 column and a specific mobile phase, the method achieved CLO elution at 10.1 minutes with high resolution from skin matrix interferences. Validation followed International Conference on Harmonization guidelines, ensuring selectivity, linearity (0.5–15.0 μg/mL), robustness, precision, accuracy, and detection limits (0.02 and 0.07 μg/mL). The method was successfully applied in in vitro skin permeation and hair follicle deposition experiments, proving its effectiveness for CLO quantification.
    Discuss this study in the Community →

    Research cited in this study

    6 / 6 results

    Related Community Posts Join

    6 / 50 results

      community New and Interesting HairLoss Studies/Papers/Reviews

      in Treatment  44 upvotes 4 years ago
      Hair loss treatments discussed include Dutasteride with Ketoconazole, tissue engineering strategies, and androgenetic alopecia therapies. Massage doubles follicular retention, improving treatment effectiveness.

      community Why is no one pointing out dandruff as the number 1 evidence/symptom of MPB?

      in Chat  55 upvotes 1 year ago
      The post discusses the theory that persistent dandruff in areas prone to hair loss could be a sign of hair follicles dying due to DHT, indicating balding. The responses vary, with some users noting improvements in dandruff and hair loss with treatments like Dutasteride, while others experienced hair loss without dandruff.

      community Androgenetic alopecia is a skin disease: DHT-mediated skin disorders

      in Research/Science  65 upvotes 1 year ago
      Dihydrotestosterone (DHT) impacts various skin conditions, including Androgenetic alopecia and seborrheic dermatitis, by causing overactivity in sebaceous glands. Topical medications Tacrolimus and Clobetasol can reduce these inflammatory conditions, and treatments like RU58841, Minoxidil, and Finasteride may also be beneficial.

      community Just find a cure already, I am totally done

      in Chat  161 upvotes 3 months ago
      The conversation is about hair loss treatments, with users recommending finasteride and minoxidil as effective solutions. Some users report side effects, but overall, the consensus is to use these treatments rather than waiting for a cure.

      community Lost density 60% all over scalp in 120 days (4months)

      in Update  10 upvotes 5 months ago
      A user lost 60% hair density in 4 months, started finasteride and microneedling, and has greasy, flaky, yellowish scalp. They are awaiting biopsy results, and another user suggested trying ketoconazole shampoo.

      community My experience and a discourse on “DUPA”

      in Chat  46 upvotes 1 year ago
      How diffuse unpatterned alopecia (DUPA) is not an invitation to self-diagnose oneself with aggressive AGA and that seeking a specialized dermatologist may help people experiencing hair loss. Treatment options discussed include topical clobetasol propionate, oral minoxidil, and discontinuing finasteride.

    Similar Research

    5 / 804 results