Chromatographic Method for Clobetasol Propionate Determination in Hair Follicles and Different Skin Layers
August 2016
in “
Biomedical Chromatography
”
TLDR A new method accurately measures clobetasol propionate in hair and skin.
The study developed a simple HPLC-UV method for quantifying clobetasol propionate (CLO) in hair follicles and skin layers, addressing the need for specific delivery in alopecia treatment. Using a RP-C 18 column and a methanol-acetonitrile-water mobile phase, the method achieved CLO elution at 10.1 min with a resolution of 2.424. It was validated according to International Conference on Harmonization guidelines, meeting criteria for selectivity, linearity (0.5–15.0 μg/mL), robustness, precision, accuracy, and detection/quantification limits (0.02 and 0.07 μg/mL). The method was successfully applied in in vitro skin permeation and hair follicle deposition experiments, proving its effectiveness.
