A Validated RP-HPLC Method to Investigate Finasteride in Human Skin After In Vitro Topically Applying Vesicular Nanocarrier

May 2014 in “ PubMed ”

Feiyue Zheng, Yuefeng Rao, Yan Lou, Xiaoyang Lu

finasteride RP-HPLC vesicular nanocarrier EclipseXDB-C18 column liposomal carriers ethosomal carriers percutaneous delivery Propecia

TLDR The method accurately measures finasteride in skin after using special carriers.

The study developed a sensitive and validated reversed-phase high performance liquid chromatography (RP-HPLC) method to measure finasteride in human cadaver skin after applying vesicular nanocarrier formulations. The method used an EclipseXDB-C18 column with a mobile phase of 75% methanol and 0.2% phosphoric acid, buffered to pH 3.0, at 40°C and a flow rate of 1 mL/min. The calibration curve was linear from 5 to 200 ng/ml with a correlation coefficient of 0.9996. The intra-assay precision was under 3.9%, and inter-assay precision was under 7.1%, with a bias range of -8.6 to 4.1%. This method was specific, accurate, and sensitive, successfully determining finasteride accumulation after in-vitro percutaneous delivery via liposomal or ethosomal nanocarriers.

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