A Validated RP-HPLC Method to Investigate Finasteride in Human Skin After In Vitro Topically Applying Vesicular Nanocarrier
May 2014
in “
PubMed
”
New to Finasteride? There is a guide in the encyclopedia. Read the guide → Studysummary This study used a newly developed RP-HPLC method to accurately measure finasteride distribution in human cadaver skin after applying different vesicular drug delivery systems. Our plain-language summary of this paper — not a Tressless recommendation.
The study developed a sensitive and validated reversed-phase high performance liquid chromatography (RP-HPLC) method to measure finasteride in human cadaver skin after applying vesicular nanocarrier formulations. The method used an EclipseXDB-C18 column with a mobile phase of 75% methanol and 0.2% phosphoric acid, buffered to pH 3.0, at 40°C and a flow rate of 1 mL/min. The calibration curve was linear from 5 to 200 ng/ml with a correlation coefficient of 0.9996. The intra-assay precision was under 3.9%, and inter-assay precision was under 7.1%, with a bias range of -8.6 to 4.1%. This method was specific, accurate, and sensitive, successfully determining finasteride accumulation after in-vitro percutaneous delivery via liposomal or ethosomal nanocarriers.
