Genetic Targeting of Lymphatic Endothelial Cells in Mice: Current Strategies and Future Perspectives
January 2024
in “
The International Journal of Developmental Biology
”
Studysummary This article reviews the diversity of lymphatic endothelial cells, related gene targeting tools, and protocols in mouse models but presents no new experimental findings. Our plain-language summary of this paper — not a Tressless recommendation.
This document discusses the diversity of lymphatic endothelial cells (LECs) and the use of mouse models for selective gene targeting of LECs. It provides an overview of commonly used LEC-specific inducible Cre lines and induction protocols, detailing essential experimental parameters and their implications. The authors describe optimized treatment regimens for targeting embryonic, postnatal, and adult LECs in organs like the mesentery and skin, highlighting the expected outcomes and limitations of each approach. The protocols aim to standardize lineage tracing and functional studies in lymphatic vascular research, offering recommendations for laboratories beginning such studies.
