Simultaneous Determination of Three Flavonoids in Rat Plasma of Masson Pinus Needles (Pinus Massoniana L.) Extracts by UHPLC-MS/MS and Application to Pharmacokinetics Studies After Oral and Transdermal Administration

August 2023 in “ Journal of Chromatographic Science ”

Chenjie Xia, Hongliu Zhu, Chang-Hui Hu, Jie Liu, Haibing Hua, Jin Yang, Zhipeng Chen, Yihong Gao

taxifolin quercetin catechin Pinus massoniana pine needle extract UHPLC-MS/MS pharmacokinetics oral administration transdermal administration

TLDR A new method accurately measures three compounds in rat plasma from pine needle extracts, aiding future research on its use for hair loss.

A UHPLC-MS/MS method was developed to quantify taxifolin, quercetin, and catechin in rat plasma after administering Pinus massoniana needle extracts, traditionally used in China to prevent hair loss. The method showed good linearity (r² > 0.99), accuracy, precision, and stability. It was successfully applied to pharmacokinetic studies in healthy rats, both orally and transdermally, providing a foundation for further research on pine needle extract as an external preparation.

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