Detection of the Nuclear Translocation of Androgen Receptor Using Quantitative and Automatic Cell Imaging Analysis

December 2024 in “ Tissue and Cell ”

Lanlan Bai, Tao Wu, Mizuki Fukasawa, Sayo Kashiwagi, Haruka Tate, Taku Ozaki, Eriko Sugano, Hiroshi Tomita, Tsuyoshi Ishii, Takuya Akashi, Tomokazu Fukuda

androgen receptor nuclear translocation dihydroxy testosterone testosterone dermal papilla cells androgenic alopecia AR DHT

TLDR A new method helps detect androgen receptor movement in cells, aiding research on hair loss treatments.

The study developed a method using quantitative and automatic cell imaging analysis to detect the nuclear translocation of the androgen receptor (AR) in immortalized dermal papilla cells (DPCs) tagged with fluorescent markers. Researchers observed AR movement from the cytoplasm to the nucleus after stimulation with dihydroxy testosterone (DHT) and testosterone, with translocation detected approximately 20 minutes post-exposure. The study analyzed 133 cells from clone 10 and 96 cells from clone 7, confirming that both DHT and testosterone induce AR nuclear translocation. This method, which overcomes previous manual limitations, could aid in screening inhibitors for AR-dependent conditions like androgenic alopecia.

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