A Correlative Fluorescent and Electron Microscopic Technique for Ultralocalization of Trichocyte Keratins

    January 2021 in “ Springer Proceedings in Materials ”
    Sailakshmi Velamoor, Sharon Lequeux, Richard Easingwood … Duane P. Harland
    Studysummary This study demonstrates a sample preparation method for wool follicles that allows for clear keratin labeling and ultrastructural preservation in microscopy, reducing common artefacts from conventional fixation techniques.
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    Research cited in this study 9

    1. Hair Follicle Differentiation-Specific Keratin Expression in Human Basal Cell Carcinoma Clinical and Experimental Dermatology · 2019
    2. Ultrastructural Localization of Hair Keratins, High Sulfur Keratin-Associated Proteins and Sulfhydryl Oxidase in Human Hair Anatomical Science International · 2016
    3. Location of Keratin-Associated Proteins in Developing Fiber Cuticle Cells Using Immunoelectron Microscopy International journal of trichology · 2010
    4. The Keratins of the Human Beard Hair Medulla: The Riddle in the Middle ˜The œjournal of investigative dermatology/Journal of investigative dermatology · 2009
    5. The Human Keratins: Biology And Pathology Histochemistry and Cell Biology · 2008
    6. Keratins of the Human Hair Follicle International review of cytology · 2005
    7. The Catalog of Human Hair Keratins Journal of Biological Chemistry · 2001
    8. Characterization of LHTric-1: A New Monospecific Monoclonal Antibody to Trichocyte Keratin Ha1 British Journal of Dermatology · 1997
    9. The Cytology and Cytochemistry of the Wool Follicle International review of cytology · 1979

    Related research 1

    1. Hard and Soft Principles Defining the Structure, Function, and Regulation of Keratin Intermediate Filaments Current Opinion in Cell Biology · 2002