A Correlative Fluorescent and Electron Microscopic Technique for Ultralocalization of Trichocyte Keratins

January 2021 in “ Springer Proceedings in Materials ”

Sailakshmi Velamoor, Sharon Lequeux, Richard Easingwood, Allan Mitchell, Jeffery Plowman, Mihnea Bostina, Duane P. Harland

trichocyte keratins keratin labeling wool follicles high-pressure frozen freeze-substituted dark-field scanning transmission electron microscopy DF-STEM ultrastructural preservation hair follicles keratin

Studysummary This study demonstrates a sample preparation method for wool follicles that allows for clear keratin labeling and ultrastructural preservation in microscopy, reducing common artefacts from conventional fixation techniques.

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This study found that beard hair medulla cells express an unexpected range of keratins, showing variability and promiscuous behavior in keratin interactions distinct from other hair follicle cells.

research The Human Keratins: Biology And Pathology

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This review summarizes the cell type distribution and functional significance of human keratins, emphasizing their roles in tumor diagnosis and potential clinical applications, and reports no new clinical findings.

research Keratins of the Human Hair Follicle

276 citations , January 2005 in “International review of cytology”

More research is needed to understand how hair keratins work and their role in hair disorders.

research The Catalog of Human Hair Keratins

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This study cataloged human type II hair keratins, detailing their expression and differentiation roles in hair follicles and comparing them with type I keratins to explore keratin-pairing principles.

research Characterization of LHTric-1: A New Monospecific Monoclonal Antibody to Trichocyte Keratin Ha1

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This study successfully developed and characterized a monospecific monoclonal antibody, LHTric-1, that specifically localizes to the pre-cortical region of the hair follicle and can aid research on hair and nail formation.

research The Cytology and Cytochemistry of the Wool Follicle

90 citations , January 1979 in “International review of cytology”

This chapter reviews the complexity of hair and wool follicle formation, emphasizing the importance of cytological studies to understand the relationship between cellular components.