Chi-miR-30b-5p Inhibits Dermal Papilla Cells Proliferation by Targeting CaMKIIδ Gene in Cashmere Goat

The study investigated the role of microRNAs (miRNAs) in hair follicle (HF) development, focusing on the dermal papilla (DP) cells which are crucial for hair formation. Through miRNA sequencing, the researchers identified 411 annotated and 130 novel miRNAs, with 29 up-regulated and 32 down-regulated during the anagen (growth) phase compared to the telogen (resting) phase. They discovered that chi-miR-30b-5p was more highly expressed during the telogen phase and found that it could inhibit DP cell proliferation by targeting the CaMKIIδ gene. The study demonstrated this effect using MTT and EdU assays on isolated DP cells, and confirmed the interaction between chi-miR-30b-5p and CaMKIIδ through dual-luciferase and western blot assays. This research provides new insights into the regulatory functions of miRNAs in HF development and the potential mechanisms controlling hair growth cycles.