CD8-Positive Lymphocytes in Graft-Versus-Host Disease of Humanized NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ Mice

The study investigated NSG-hu-BLT mice, which are immunocompromised mice engrafted with human fetal liver and thymus, to model human immune system functions. Four mice developed alopecia, pruritus, and lethargy after HSPC engraftment, and histopathology revealed signs of graft-versus-host disease (GvHD), including keratinocyte and hepatocyte cell death associated with CD8+ T lymphocytes. This highlighted the role of CD8+ T cells in GvHD progression and suggested that while NSG-hu-BLT mice are valuable for studying GvHD, their susceptibility to the disease might limit their utility in other biomedical research areas.