Serum and Liver Tissue Biotinidase Enzyme Activity in Rats Administered Valproic Acid

The study investigated the effects of valproic acid (VPA) on biotinidase enzyme activity in rats, focusing on its potential link to alopecia. Four groups of 15 male Wistar rats were administered varying doses of VPA (200, 400, and 600 mg/kg/day) or distilled water for 60 days. Results showed a dose-dependent increase in alopecia, with 6.6% in the lowest dose group and 26.6% in the highest. Both liver and serum biotinidase enzyme activities decreased with higher VPA doses, suggesting a correlation between reduced biotinidase activity and alopecia. The study concluded that biotin supplementation (10 mg/day) might prevent alopecia in patients undergoing VPA therapy.