Development and Validation of Androgen-Induced Hair Loss and Anagen Induction Mouse Models for Pharmacological Evaluation of Anti-Androgenic Agents

The study validated androgen-induced hair loss and anagen induction mouse models for evaluating anti-androgenic agents in Androgenetic alopecia (AGA). Subcutaneous administration of 30 mg/kg of DHT was found optimal for inducing hair loss without skin lesions. Both Minoxidil and Pyrilutamide significantly promoted hair growth in these models by decreasing AR protein levels and enhancing the Wnt/β-Catenin signaling pathway. These models are effective for studying the efficacy and mechanisms of new AGA treatments.