Technical Advancement and Biomedical Application of Multiphoton Excitation Microscopy

    January 2019 in “ The Review of Laser Engineering
    Yusuke Oshima, Noriko Sagawa, Takahiro Hiratsuka, T.G. Etoh, Masahumi Inomata
    TLDR Multiphoton excitation microscopy is a promising tool for deep tissue imaging and clinical applications.
    The document discussed the use of multiphoton excitation microscopy as a promising technique for intravital imaging in biology and clinical medicine. This nonlinear optical phenomenon allowed for deep tissue analysis and label-free imaging of collagen. The study specifically used this technique to investigate chemotherapy-induced alopecia in mice, observing changes in vascular structure and hair papillae after treatment with cyclophosphamide. The advancements in this microscopy technique suggested its potential as a practical tool for both bioimaging analysis and future clinical applications.
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