Long-Term Intravital Imaging of the Cornea, Skin, and Hair Follicle by Multiphoton Microscope

January 2019 in “ Methods in molecular biology ”

Yueh‐Feng Wu, Hsin‐Yuan Tan, Sung‐Jan Lin

multiphoton microscopy hair follicles fluorescent proteins extracellular matrix stem cell research

TLDR Multiphoton microscopy can effectively image live cells in cornea, skin, and hair follicles over time.

The study demonstrated the use of multiphoton microscopy for long-term imaging of cells in live animals, specifically focusing on the cornea, skin, and hair follicles in transgenic mice. This technique allowed for cell imaging and tracing at a single-cell resolution, utilizing fluorescent proteins to tag specific cell populations. The method provided insights into cell dynamics and structural information from the extracellular matrix, offering advantages over conventional histological analysis in stem cell research.

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