Long-Term Intravital Imaging of the Cornea, Skin, and Hair Follicle by Multiphoton Microscope

January 2019 in “ Methods in molecular biology ”

Yueh‐Feng Wu, Hsin‐Yuan Tan, Sung‐Jan Lin

multiphoton microscopy hair follicles fluorescent proteins extracellular matrix stem cell research

TLDR Multiphoton microscopy can effectively image live cells in cornea, skin, and hair follicles over time.

The study demonstrated the use of multiphoton microscopy for long-term imaging of cells in live animals, specifically focusing on the cornea, skin, and hair follicles in transgenic mice. This technique allowed for cell imaging and tracing at a single-cell resolution, utilizing fluorescent proteins to tag specific cell populations. The method provided insights into cell dynamics and structural information from the extracellular matrix, offering advantages over conventional histological analysis in stem cell research.

View this study on link.springer.com →

Discuss this study in the Community →

Research cited in this study

3 / 3 results

research Intravital Imaging of Hair Follicle Regeneration in the Mouse

56 citations , June 2015 in “Nature Protocols”

Two-photon microscopy helps observe hair follicle stem cell behaviors in mice.

research Live Imaging of Stem Cell and Progeny Behavior in Physiological Hair-Follicle Regeneration

305 citations , June 2012 in “Nature”

Hair regeneration needs dynamic cell behavior and mesenchyme presence for stem cell activation.

research Molecular Dissection of Mesenchymal–Epithelial Interactions in the Hair Follicle

417 citations , September 2005 in “PLoS biology”

Understanding gene expression in hair follicles can reveal insights into hair growth and disorders.

Similar Research

5 / 169 results

research Application of Multiphoton Microscopy in Dermatological Studies: A Mini-Review

58 citations , November 2013 in “Journal of Innovative Optical Health Sciences”

Multiphoton microscopy is a promising tool for detailed skin imaging and could improve patient care if its challenges are addressed.

research Two-Photon Microscopy for Intracutaneous Imaging of Stem Cell Activity in Mice

16 citations , September 2016 in “Experimental Dermatology”

Two-photon microscopy effectively tracks live stem cell activity in mouse skin with minimal harm and clear images.

research Fluorescence Microscopy: An Outline of Hardware, Biological Handling, and Fluorophore Considerations

97 citations , December 2021 in “Cells”

Designing effective fluorescence microscopy experiments requires careful consideration of hardware, biological models, and imaging agents.

research Proceedings of the Ninth World Congress for Hair Research 2015

January 2017 in “Journal of Investigative Dermatology Symposium Proceedings”

The 2015 Hair Research Congress concluded that stem cells, maraviroc, and simvastatin could potentially treat Alopecia Areata, topical minoxidil, finasteride, and steroids could treat Frontal Fibrosing Alopecia, and PTGDR2 antagonists could also treat alopecia. They also found that low-level light therapy could help with hair loss, a robotic device could assist in hair extraction, and nutrition could aid hair growth. They suggested that Alopecia Areata is an inflammatory disorder, not a single disease, indicating a need for personalized treatments.

research Stem Cells in the Body

November 2017

Stem cells regenerate tissues and their behavior varies by environment, suggesting the hematopoietic system model may need revision.