TLDR Two-photon microscopy helps observe hair follicle stem cell behaviors in mice.
This document is a collection of protocols and methods for using intravital imaging to study hair follicle regeneration in mice. The authors describe the use of two-photon laser-scanning microscopy (TPLSM) to capture behaviors such as apoptosis, proliferation, and migration of hair follicle stem cells. They also discuss the challenges of visualizing hair follicles in the mouse skin and provide recommendations for optimal skin preparation and mounting. The authors highlight the advantages of intravital imaging for addressing outstanding questions in the stem cell field.
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