Fluorescence Microscopy: An Outline of Hardware, Biological Handling, and Fluorophore Considerations
December 2021
in “
Cells
”
TLDR Designing effective fluorescence microscopy experiments requires careful consideration of hardware, biological models, and imaging agents.
The review discussed the evolution and advancements in fluorescence microscopy, focusing on hardware improvements, biological handling, and imaging agents. It highlighted the transition from widefield epifluorescence to advanced systems like confocal and multiphoton microscopy, which improved spatial resolution and tissue penetration. Techniques such as FLIM, FRET, and FRAP were used to study cellular functions, while light-sheet and super-resolution microscopy offered efficient imaging of larger samples. The document emphasized the importance of interdisciplinary collaboration and optimizing experimental design to enhance imaging outcomes. It also explored the use of fluorescent proteins and graphene quantum dots for non-invasive live cell imaging, despite challenges like phototoxicity and high background fluorescence. The review underscored the potential of these technologies in advancing cellular and molecular biology research.
