Multiphoton Microscopic Imaging of In Vivo Hair Mouse Skin Based on Two-Photon Excited Fluorescence and Second Harmonic Generation
September 2011
in “
Scanning
”
Studysummary This study found that multiphoton microscopy effectively visualizes the microstructure of in vivo mouse skin, offering a clear view of various skin layers and components like corneocytes and collagen fibers.
Our plain-language summary. Not medical advice or a treatment recommendation. Consult a qualified healthcare professional before changing treatment. Full disclaimer
The study explored the use of multiphoton microscopy (MPM) for imaging in vivo hair mouse skin, utilizing two-photon excited fluorescence (2PEF) and second harmonic generation (SHG). The research demonstrated that MPM could effectively reveal the microstructure of the stratum corneum, stratum spinosum, and dermis, allowing for the clear identification of components such as corneocytes, spinosum cells, collagen fibers, and hair follicles. The study involved five Kunming white mice and highlighted MPM's potential as a valuable tool for investigating skin physiological and pathological states in a non-invasive manner.
